EŠect of a-Tocopherol on the Oxidative Modiˆcation of Apolipoprotein E in Human Very-low-density Lipoprotein
نویسندگان
چکیده
Abbreviations : apo, apolipoprotein; CE-OOH, cholesteryl ester hydroperoxides; RP-HPLC, reversed-phase high-performance liquid chromatography; LDL, low-density lipoprotein; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TBARS, 2-thiobarbituric acid-reactive substances; TBS, Tris-HCl-buŠered saline; a-Toc, a-tocopherol; VLDL, very-low-density lipoprotein Biosci. Biotechnol. Biochem., 67 (2), 402–405, 2003
منابع مشابه
In Vitro Effect of ?-Tocopherol, Ascorbic Acid and Lycopene on Low Density Lipoprotein Glycation
Nonenzymatic glycation of low density lipoprotein (LDL) is a reaction of glucose and other reducing sugars with apolipoprotein B100 (apo-B100) lysine residues. In diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. The objective of this study was to investigate in vitro effects of ?-tocopherol, ascorbic acid and lycopene on LDL glycatio...
متن کاملIn Vitro Effect of ?-Tocopherol, Ascorbic Acid and Lycopene on Low Density Lipoprotein Glycation
Nonenzymatic glycation of low density lipoprotein (LDL) is a reaction of glucose and other reducing sugars with apolipoprotein B100 (apo-B100) lysine residues. In diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. The objective of this study was to investigate in vitro effects of ?-tocopherol, ascorbic acid and lycopene on LDL glycatio...
متن کاملThe Effect of ? -Tocopherol on Copper Binding to Low Density Lipoprotein
The oxidative modification of low density lipoprotein (LDL) may play an important role in atherogenesis. Antioxidants that can prevent LDL oxidation may act as antiatherogens. Our understanding of the mechanism of LDL oxidation and factors that determine its susceptibility to oxidation is still incomplete. Copper is a candidate for oxidizing LDL in atherosclerotic lesions. The binding of copper...
متن کاملThe Effect of ? -Tocopherol on Copper Binding to Low Density Lipoprotein
The oxidative modification of low density lipoprotein (LDL) may play an important role in atherogenesis. Antioxidants that can prevent LDL oxidation may act as antiatherogens. Our understanding of the mechanism of LDL oxidation and factors that determine its susceptibility to oxidation is still incomplete. Copper is a candidate for oxidizing LDL in atherosclerotic lesions. The binding of copper...
متن کاملApolipoprotein E Polymorphism in an Iranian Hypercholestrolemic Population
Apolipoprotein E (apo E) is a structural constituent of several serum lipoprotein classes. It plays an important role in lipid metabolism by acting as a ligand for low-density lipoprotein (LDL) and chylomicron remnant receptors. Three common alleles called e2, e3 and e4 have been described, which code for three protein isoforms (E2, E3 and E4). The polymorphism is clinically significant, and it...
متن کامل